Isozymes from Conchocelis of <i>Porphyra</i> Species

Kinetic patterns of glucoamylase isozymes isolated from Aspergillus species.

Patterns of action of glucoamylase isozymes from Aspergillus species on glycogen.

Glucoamylase isozymes from black Aspergillus species have been freed of all traces of alpha-amylase by chromatography on Bio-Gel P-100, as evidenced by limited hydrolysis of oxidized amylose. Glucoamylase I retains its ability to hydrolyze rabbit-liver glycogen rapidly. By contrast, glucoamylase II hydrolyzes glycogen slowly, and addition of alpha-amylase to glucoamylase II does not enhance its...

Peroxidase Isozymes from Horseradish Roots

Seven peroxidase isozymes were isolated from horseradish roots and purified to homogeneity as ascertained by chromatography, ultracentrifugation, and polyacrylamide disk electrophoresis. Purification procedures included ammonium sulfate precipitation and column chromatography on carboxymethyl and diethylaminoethyl cellulose. Peroxidase activity in the seven isozymes accounted for 86% of the act...

Responses of chlorophyll a content for conchocelis phase of alaskan porphyra (bangiales,rhodophyta) species to environmental factors

Investigations were performed on variations of photosynthetic pigment in conchocelis of Alaskan Porphyra species, P. abbottae (Pa), P. pseudolanceolata (Pe), P. pseudolinearis (Pi) and P. torta (Pt), in response to environmental variables. Conchocelis were cultured under varying conditions of irradiance (0, 10, 40 and 160 μmol photons m-2s-1), nutrient concentration (0, f/4, f/2 and f) for up t...

Pyruvate Kinase Isozymes from Rat Tissues

Developmental and aging patterns of pyruvate kinase (PK) isozymes (PK 1, PK 2, PK 3, and PK 4) from rat liver, kidney, heart, and skeletal muscle were studied by electrophoretic, chromatographic and immunochemical techniques. During the first 2 weeks of postnatal life a decline of total PK activity occurred in liver, kidney, and heart followed by an increase to adult or higher than adult levels...